Prototype stage

Gene detail

Read-only gene view with summary, GO, UniProt, NCBI, and representative sequence links.

GRCh38.p14 + GENCODE Release 50local-onlyPDO SQLite primaryread-only query modesqlite3 fallback available

Gene detail

RPA1

protein_coding 17 1,829,702 - 1,900,082 PDO SQLite primary read-only query mode

Overview Summary GO UniProt Representative Transcripts FASTA ClinVar disease associations

Overview

Gene ID
ENSG00000132383

Gene type
protein_coding

Chromosome
17

Coordinates
1,829,702 - 1,900,082

Strand
+

Status
not available

NCBI summary UniProt GO Transcript FASTA Protein FASTA

Aliases

6117 CCDS11014 ENST00000254719.10 HSSB NM_002945 NM_002945.5 P27694 REPA1 RF-A RP-A RPA70 replication protein A1 (70kD) replication protein A1, 70kDa

Summary

GENCODE gene_type=protein_coding; HGNC symbol=RPA1; HGNC name=replication protein A1; alias_count=14; RefSeq=NM_002945; UniProt=P27694; MANE Select=ENST00000254719.10,NM_002945.5

Source: GENCODE + HGNC complete set

6117 • protein-coding

This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]

NCBI Gene

UniProt

P27694 • reviewed

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:17596542, PubMed:27723717, PubMed:27723720). Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Also plays a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. Plays a role in telomere maintenance (PubMed:17959650, PubMed:34767620). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). RPA stimulates 5'-3' helicase activity of the BRIP1/FANCJ (PubMed:17596542)

Replication protein A 70 kDa DNA-binding subunit · Nucleus; Nucleus, PML body

GO annotations

Biological process

GO:0006974 DNA damage response (IMP)
GO:0006310 DNA recombination (IEA)
GO:0006310 DNA recombination (TAS)
GO:0006281 DNA repair (IEA)
GO:0006281 DNA repair (IMP)
GO:0006281 DNA repair (IDA)
GO:0006260 DNA replication (IEA)
GO:0006260 DNA replication (IMP)
GO:0006260 DNA replication (NAS)
GO:0006260 DNA replication (IBA)

+ 20 more

Cellular component

GO:0005662 DNA replication factor A complex (IEA)
GO:0005662 DNA replication factor A complex (IDA)
GO:0005662 DNA replication factor A complex (IPI)
GO:0005662 DNA replication factor A complex (IDA)
GO:0005662 DNA replication factor A complex (IDA)
GO:0005662 DNA replication factor A complex (IPI)
GO:0005662 DNA replication factor A complex (IBA)
GO:0016605 PML body (IDA)
GO:0016605 PML body (IEA)
GO:0016605 PML body (IDA)

+ 94 more

Molecular function

GO:0003677 DNA binding (IEA)
GO:0098505 G-rich strand telomeric DNA binding (IDA)
GO:0098505 G-rich strand telomeric DNA binding (IDA)
GO:0098505 G-rich strand telomeric DNA binding (IMP)
GO:0003682 chromatin binding (IEA)
GO:0140463 chromatin-protein adaptor activity (IDA)
GO:0003684 damaged DNA binding (IDA)
GO:0003684 damaged DNA binding (IDA)
GO:0003684 damaged DNA binding (IDA)
GO:0003684 damaged DNA binding (IBA)

+ 52 more

Representative

Representative transcript
ENST00000254719

Representative protein
ENSP00000254719.4

Representative type
CCDS

Candidate count
2

GENCODE Release 50 annotation GTF · transcript.tag=CCDS; transcript_support_level=1

Transcript FASTA Protein FASTA

Transcripts

Transcript ID	Name	Type	Status	Protein	Location
ENST00000571058	RPA1-203	protein_coding	not available	ENSP00000461733.1	17:1,829,702 - 1,872,483 +
ENST00000852055	RPA1-208	protein_coding	not available	ENSP00000522114.1	17:1,829,950 - 1,900,082 +
ENST00000852056	RPA1-209	protein_coding	not available	ENSP00000522115.1	17:1,829,950 - 1,900,082 +
ENST00000852057	RPA1-210	protein_coding	not available	ENSP00000522116.1	17:1,829,950 - 1,900,082 +
ENST00000852058	RPA1-211	protein_coding	not available	ENSP00000522117.1	17:1,829,950 - 1,900,082 +
ENST00000852059	RPA1-212	protein_coding	not available	ENSP00000522118.1	17:1,829,950 - 1,900,082 +
ENST00000852060	RPA1-213	protein_coding	not available	ENSP00000522119.1	17:1,829,950 - 1,900,082 +
ENST00000852061	RPA1-214	protein_coding	not available	ENSP00000522120.1	17:1,829,950 - 1,900,082 +
ENST00000852062	RPA1-215	protein_coding	not available	ENSP00000522121.1	17:1,829,950 - 1,900,082 +
ENST00000852063	RPA1-216	protein_coding	not available	ENSP00000522122.1	17:1,829,950 - 1,900,082 +
ENST00000852064	RPA1-217	protein_coding	not available	ENSP00000522123.1	17:1,829,950 - 1,900,082 +
ENST00000933416	RPA1-218	protein_coding	not available	ENSP00000603475.1	17:1,829,950 - 1,900,082 +
ENST00000933417	RPA1-219	protein_coding	not available	ENSP00000603476.1	17:1,829,950 - 1,900,082 +
ENST00000933418	RPA1-220	protein_coding	not available	ENSP00000603477.1	17:1,829,950 - 1,900,082 +
ENST00000933419	RPA1-221	protein_coding	not available	ENSP00000603478.1	17:1,829,950 - 1,900,082 +
ENST00000933420	RPA1-222	protein_coding	not available	ENSP00000603479.1	17:1,829,950 - 1,900,082 +
ENST00000962481	RPA1-223	protein_coding	not available	ENSP00000632540.1	17:1,829,950 - 1,900,082 +
ENST00001123225	RPA1-232	protein_coding	not available	ENSP00000793030.1	17:1,829,950 - 1,900,082 +
ENST00001143719	RPA1-233	protein_coding	not available	ENSP00000802737.1	17:1,829,950 - 1,900,082 +
ENST00001045462	RPA1-226	nonsense_mediated_decay	not available	ENSP00000715279.1	17:1,829,970 - 1,899,552 +

FASTA

FASTA output is generated by backend query; the raw FASTA path is not exposed.

Open transcript export Open protein export

ClinVar disease associations

ClinVar disease associations: 0

ClinVar gene-disease tables are missing. Build the candidate database first.